PowerPoint for my McNair Conference presentation on the effects of TPP-1 enzyme therapy on Neural Progenitor Cells
My biggest contribution to this project was improving how the cells are quantified. I found an open-source software called Cellprofiler to perform the counts. Using this program, I designed pipelines to separate positive from negative nuclei.
I had the program target certain wavelengths emitted by GFAP positive human neural progenitors.
CLN2-Presentation2-Diane-McNair-1-1Summer Research 2020
For Summer I’m assisting Dr. James Munoz at NOVA Southeastern University on the effects of TPP-1 enzyme replacement therapies on brain cells. I’ll hopefully be going into the lab to run some protocols in person early August. For now, I have been helping with the cell counts of the work they have done so far. I sourced out an open source program called CellProfiler for cell counting. It has proved to be an amazing program. You can check it out here https://cellprofiler.org/ I do not know Python and ReGex and still find the program easy to use. I want to be able to really use all of it’s features so I started an EdX Python Basics for Data Science course to better understand. Here’s a screen shot of an analysis I made with it that was under 1% error. It took less than 5 seconds to process after I perfected the settings.
Spring Research 2020
I’m doing undergraduate research at the University of Florida’s Davie Extension Department of Microbiology and Cell Science with Dr. Ulirich Stingl Cultivating Sar 11 bacteria.
Covid and Spring Research summed up in one photo, lol.
I managed to cobble together a presentation on my SAR 11 cultivation work. Unfortunately, nearly all the data was in a computer locked away at UF. I will always save everything to the cloud to avoid this in the future.
Sarr-11-Culturing-power-pointFall Research of Polynucleobacter and it’s the population in different salinities in the Everglades.
The bulk of my work during this internship was using Fluorescence in Situ Hybridization to photograph and count microbial communities in the Everglades Water Column. Here is a collection of some of the thousands of images I took. I became a bit of an expert in the lab running these protocols.
